Development of confirmatory testing strategies for suspect false PRRS-positive reactors in diagnostic serology
نویسندگان
چکیده
The commercial ELISA kit (IDEXX HerdCheck PRRS) has been the main serodiagnostic tool for PRRS virus (PRRSV) in North America and many pig producing regions throughout the world. Although the kit has been mostly a reliable test for detecting PRRSV exposure at the herd level, the specificity of the kit has been frequently challenged by the occurrence of unexpected false positive results at varying rates. An indirect fluorescent antibody (IFA) test has been commonly employed to confirm or disprove the suspect false positive reactivity in the ELISA. However, the reliability of test results have been often questioned due to the subjectivity in determination of test result and perceived lower sensitivity. The current study was conducted first to develop immunoassays (Western
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